The RP method is widely performed using capillary tubes to draw blood from the retrobulbar venous sinus. There are multiple sites for blood collection in mice, including the retroorbital plexus (RP), lateral tail vein, saphenous vein, heart, and facial vein (FV), also known as the submandibular vein. When researchers plan to undertake blood collection, they should consider not only the methods but also animal welfare, with the aim of minimizing pain, distress, tissue damage, and lasting harm to the animal. The quantity and quality of blood extracted varies according to the experimental aim and study design, which can affect experimental results.
MIX NINE BAE YOON JUNG SERIAL
With respect to animal ethics, we suggest that the FV method, a simple and fast technique that can easily be performed by both experienced and novice researchers, is suitable for serial blood collection.īlood collection is one of the basic methods used to obtain the experimental results from in vivo studies. White blood cells were infiltrated in RP areas with severe tissue damage and inflammation. The proportion of granulocytes and monocytes, such as macrophages in the peripheral blood and spleen, was decreased in mice subjected to FV methods compared with that in mice subjected to RP methods in both experienced and novice groups. Corticosterone concentrations in the peripheral blood were decreased in mice subjected FV methods compare with those subjected to RP methods. We found that the FV methods reduced the collection time, pain, distress, tissue damage and lasting harms without anesthesia. After blood collection, all mice were evaluated by corticosterone concentrations for stress, hematological, immunological, and histological analyses. The experienced and novice groups collected up to 0.4 mL of blood via the RP and FV methods every second day for 2 weeks. To suggest simple methods that minimize pain during serial blood collection, we compared the retroorbital plexus (RP) and facial vein (FV) blood collection methods performed by both experienced and novice groups. Various comparative studies of blood collection methods have been reported however, there are no comparative studies on serial blood collection considering animal ethics. Blood collection methods that reduce pain, injury, and stress in experimental animals are important with regard to animal ethics. There has been an outpouring of emotion on social media as news of Dong Yoon’s passing spread.Blood collection is an important procedure used in animal experiments. On social media, Dong Yoon operated a private Twitter account and an Instagram account with no posts. When I was on stage, it was a great feeling… It’s amazing to be an entertainer.” Dong Yoon told Top Star News in an interview in May 2018 about his time on the show saying, “It was so hard to practice and I couldn’t sleep at the time, but I was so proud of it after I came out. Dong Yoon made it all the way to the show’s final episode which aired in January 2018.
He would join the band after his elimination from the show. Mix Nine is a South Korean survival-based reality show that Dong Yoon made his first appearance on in December 2017 rather than join up with the band Spectrum. We express our condolences for his final path.” According to the wishes of the family of the deceased, his funeral will be quietly. We are so heartbroken and sorry to be conveying this sad news to Dong Yoon’s fans, who sincerely loved and generously encouraged him. His family members, Spectrum members, and all the staff at WNNY Entertainment are grieving with a heavy heart over this sudden sad news. follow us on twitter: /dongyooons /changyoontwt T05:53:27.000ZĪ longer statement from WYNN Entertainment read, “Our agency’s artist and Spectrum member Kim Dong Yoon left our side on July 27. Mixnine WYNN Kim Dongyoon Self Cam before audition DO NOT REUPLOAD OR EDIT.
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